The role of the histone methyltransferase egg/dSETDB1 in Drosophilia development and oogenesis. Emily A Clough

ISBN: 9780549985570

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117 pages


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The role of the histone methyltransferase egg/dSETDB1 in Drosophilia development and oogenesis.  by  Emily A Clough

The role of the histone methyltransferase egg/dSETDB1 in Drosophilia development and oogenesis. by Emily A Clough
| NOOKstudy eTextbook | PDF, EPUB, FB2, DjVu, talking book, mp3, ZIP | 117 pages | ISBN: 9780549985570 | 4.40 Mb

Histone methylation is an important feature of both heterochromatin and euchromatic gene regulation. The Drosophila melanogaster gene eggless (egg) encodes a histone methyltransferase similar to human SETDB1 and was discovered in an EMS mutagenesisMoreHistone methylation is an important feature of both heterochromatin and euchromatic gene regulation. The Drosophila melanogaster gene eggless (egg) encodes a histone methyltransferase similar to human SETDB1 and was discovered in an EMS mutagenesis screen for lethal and female-sterile mutations uncovered by Df(2R)DII-MP.

I have characterized the role of egg during Drosophila development and oogenesis. Loss of egg results in reduced viability that is enhanced for males compared to females. egg adult flies display several mutant phenotypes including short life span, wing defects, mobility defects, and female sterility. Ovaries from females bearing strong egg alleles arrest early in oogenesis. egg mutant somatic cells have reduced proliferation and both germ and somatic cells undergo apoptosis. I tested the hypothesis that egg functions as a histone 3, lysine 9 (H3K9) histone methyltransferase (HMT), similar to SETDB1.

I observed that egg is required for trimethylation of H3K9 in both germ and somatic cells of the ovary. Clonal analysis was performed to determine whether germ and somatic cells of the ovary intrinsically require eggs HMT activity and what aspects of oogenesis are impacted by loss of egg. Clonal analysis experiments revealed that egg is required in germ cells for viability between stage 4 and 6 of oogenesis and in the germ stem cell for its maintenance.

Examining the ovaries from females bearing weak egg alleles suggests that egg is also required late in oogenesis for degeneration of nurse cells. Loss of egg in the prefollicular somatic cells of the germarium leads to apoptosis of these cells. These results suggest that H3K9 methylation catalyzed by egg is required for many critical biological processes during Drosophila development and oogenesis and that egg may function in multiple genetic pathways.



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